Figure 2.

Expression of PuSWEET15 in NG and BNG fruits and its subcellular localization. A, Relative expression of PuSWEET15 during NG and BNG fruit development as determined by RT-qPCR. Fruit samples were collected in 2014. Numbers under the x axes indicate the DAFB. Three biological replicates were analyzed, and the error bars represent the se. Asterisks indicate significant difference as determined by Student’s t test (**P < 0.01). B, Subcellular localization of PuSWEET15. 35S:GFP-PuSWEET15 was transiently expressed in protoplasts of maize leaves. Transient expression of GFP alone (35S:GFP) was used as a control. FM4-64 was used as a plasma membrane marker. Scale bars = 5 μm.