Table 1. Enzyme kinetics of the five recombinant PpAAT1s using 4-hydroxydecanoyl-CoA as substrate.
Data are presented as means ± se. Letters indicate significant differences at P ≤ 0.05 (n = 6) as determined using ANOVA followed by Fisher’s lsd test.
| Enzymes | Km | Kcat | Kcat/Km |
|---|---|---|---|
| mm | s–1 | ||
| Type I | 6.5211 ± 0.4321 b | 0.1552 ± 0.0112 b | 0.0232 ± 0.0011 b |
| Type II | 2.4144 ± 0.1516 a | 2.2411 ± 0.0991 a | 0.9507 ± 0.0661 a |
| Type III | 6.3314 ± 0.5611 b | 0.1446 ± 0.0191 b | 0.0224 ± 0.0031 b |
| Type IV | 7.0852 ± 0.6911 b | 0.1720 ± 0.0160 b | 0.0220 ± 0.0013 b |
| Type V | 6.2014 ± 0.4952 b | 0.1730 ± 0.0120 b | 0.0271 ± 0.0014 b |