Figure 1.

Phenotypes of wild-type, erdj3b-1, and erdj3b-2 plants grown at 29°C. A, Inflorescences of wild-type (WT), erdj3b-1 (3b-1), and erdj3b-2 (3b-2) plants grown at 29°C. Seed numbers per silique are shown in Supplemental Figure S1. Bar = 10 mm. B, Flowers observed by stereomicroscopy. Bar = 0.5 mm. C, Mature anthers observed by stereomicroscopy. Note that erdj3b mutant anthers dehisce normally and pollen grains are observed on the anthers. Bar = 100 μm. D, Self-pollinated stigmas stained by DiOC₂ and observed using differential interference contrast microscopy. Pollen grains were visualized by DiOC₂ staining, which stains pollen exine. Arrows indicate pollen grains on the mutant stigmas (Regan and Moffatt, 1990). Bar = 100 μm. E, Number of pollen grains on stigmas of wild-type (n = 14), erdj3b-1 (n = 16), and erdj3b-2 (n = 15) plants grown at 29°C. Each dot shows the number of pollen grains on each stigma; the circle indicates an outlier. Statistical differences were calculated using the Tukey-Kramer method. Different letters indicate statistically significant differences between results at P < 0.05.