Figure 3.

Developing anthers of the erdj3b mutant grown at 29°C show tapetal hypertrophy. Semithin sections of anthers prepared from wild-type (A, C, E, and G) and erdj3b-1 (B, D, F, and H) plants grown at 29°C, stained with Toluidine Blue, and observed by differential interference contrast microscopy are shown. Sections of anthers at stage 5 (A and B), stage 7 (C and D), stage 8 (E and F), and stage 9 (G and H) are shown. E, Epidermis; En, endothecium; ML, middle layer; MMC, microspore mother cells; Msp, microspores; T, tapetum cell; Td, tetrads. Arrowheads in B indicate aberrant middle layer-like cells. In H, arrows indicate collapsed pollen grains. Bars = 30 μm.