Membrane water permeability of root cortex cells and leaf mesophyll cells. Lpc (A–C) and the osmotic water permeability coefficient of leaf mesophyll cell protoplasts (Pos; d–F) were measured in wild-type (WT), PIP2;5 OE-4, PIP2;5 OE-13, and pip2;5 KO lines using 1- and 3-week-old plants, respectively, grown under hydroponic conditions. Individual data dots are shown and data are also expressed as the mean ± se of 10–15 cells from three to five plants for the Lpc and >30 protoplasts from two plants for the Pos. Significant differences among the treatments are indicated by *P < 0.05, **P < 0.01, and ***P < 0.001. ns, Not significantly different (P > 0.05).