Figure 1:

Schematic diagram of the thermodynamic cycle for the single decoupling method. The binding free energy between receptor R and ligand L stands for ΔG^o. The binding free energy is calculated by $\text{Δ}{G}^o$. The binding free energy is calculated by. $\text{Δ}{G}^o=-\text{Δ}{G}_{\text{restr}}^{RL}-\text{Δ}{G}_{\text{decoupl}}^{RL}+\text{Δ}{G}_{\text{restr}}^{R+L}$.