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. 2020 Apr 1;10:393. doi: 10.3389/fonc.2020.00393

Figure 2.

Figure 2

Inhibition of Akt signaling in AML by ISC-4. (A) Western blot analysis of AML cells exposed to increasing concentrations of ISC-4 or GDC-0941 (1 μM) for 24 h. GAPDH was used as a loading control. (B) Densitometric quantification of western blot bands. (C) Detection of PI3K activation by flow cytometry in bulk primary human AML cells (n = 3). (D,E) Flow cytometric detection of phospho-Akt (p-Akt) in CD34+ primary human AML cells (n = 5) after treatment with DMSO or ISC-4 (1–10 μM). Results are mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ****P < 0.0001 was assessed by one-way ANOVA.