Figure 3.

Characterization of cardiogenic vectors (GATA4, MEF2C, and TBX5) in complex with CiCMC‐NPs by RT‐PCR, Western blot, FACS, and confocal microscopy. A) Zeta‐potential due to the formation of CiCMC‐NPs. B,a) Scheme of interaction between cationic CMC‐NPs and anionic GMT pDNA. C) Maps of expression vectors generated by recombinant PCR methods. D) FACS analysis of the efficiency of pEGFP‐encoding vectors. E) Transfection efficiency of NHDFs using CiCMC‐NPs, at the mRNA and protein levels, as determined by confocal microscopy. Vector: a) GATA4, b) MEF2C, and c) TBX5.