Figure 2.
(A)B6129F1 immunocompetent mice bearing RPP tumors on their flank were treated with SRA737 (100mg/kg, 3/7 days or 5/7 days) and/or anti-PD-L1 (300μg, 1/7 days) (n = 10 per group). Tumor growth curves are shown as the mean tumor volume +/− standard error of the mean (SEM).
(B)Immunoblot analysis of tumors resected at Day 7 from a cohort of mice treated with vehicle, SRA737 (5/7), anti-PD-L1 or SRA737 (5/7) + anti-PD-L1. STING pathway activation was determined by immunoblotting for pSTING_S366, total STING, pTBK1_S172, total TBK1, cGAS, pIRF3_S396, and total IRF3.
(C) Quantitative PCR (qPCR) measurement of IFNβ, CXCL10 and CCL5 mRNA expression in SCLC tumors resected as described in B.
Data presented as mean ± SD and p values by t-test * p<0.05, **p<0.01, ***p<0.001.