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. 2020 Mar 24;11(3):345. doi: 10.3390/genes11030345

Figure 1.

Figure 1

Phylogenetic analysis and expression pattern of MLK3. (a) Phylogenetic analysis of the four MLKs using DNAMAN (version 7.0, Lynnon Corporation, Quebec, Canada). (b) Expression analysis of MLK3 transcript by reverse transcription-polymerase chain reaction (RT-PCR). Total RNA of roots and leaves was isolated from 2-week-old plants, and total RNA of stems and flowers was from mature plants. POLYUBIQUITIN 10 (At4g05320) was used as an internal standard. (c) Subcellular localization of the MLK3-GFP fusion protein in tobacco leaf. Agrobacterium expressing 35S::MLK3-GFP or 35S::GFP was infiltrated into tobacco leaves separately. Transiently expressed GFP was imaged by confocal fluorescence microscopy 36 hours after the infiltration. Nucleus was indicated by DAPI staining. Bars = 10 µm.