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. 2020 Mar 24;11(3):345. doi: 10.3390/genes11030345

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Disruption of MLK3 caused early-flowering. (a) Representative image of 24-day-old plants grown under long-day (LD). Bar = 2 cm. (b) Flowering time analysis. Days after germination (DAG) were measured as the number of days from germination to the emergence of the first flower under LD. Plants (n ≥ 30) were counted from three independent replicates. * indicated the significant difference from wild type (Student’s t-test, p < 0.05). (c) Representative image of mature plants (five-week-old) under LD. Arrowhead indicated young siliques. Bar = 1 cm. (d) Relative expression level of FLOWERING LOCUS C (FLC) by qRT-PCR and POLYUBIQUITIN 10 was used as the internal control. Three biological replicates were performed. * indicated the significant difference from wild type (Student’s t-test, p < 0.05).