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. 2020 Mar 24;11(3):345. doi: 10.3390/genes11030345

Figure 5.

Figure 5

Analysis of leaf growth and flowering time in mlk3 plants expressing 35S::MLK3 or 35S::MLK3(K146R). (a) Transcriptional analysis of MLK3 by semi-quantitative RT-PCR. Total RNA was isolated from 10-day-old plants of the indicated genotypes. Line 8 and Line 6 were analyzed for the transgenic plants of 35S::MLK3/mlk3 and 35S::MLK3(K146R)/mlk3, respectively, and Actin 2 (At3g18780) was used as internal control. (b) Comparison of the rosette leaf number of the transgenic mlk3 plants expressing 35S::MLK3 or 35S::MLK3(K146R). Transgenic Line 8 and Line 6 were used for 35S::MLK3/mlk3 and 35S::MLK3(K146R)/mlk3, respectively. * indicated a significant difference from wild type (Student’s t-test, p < 0.05). Leaf with petiole was counted with at least ten plants for individual genotype and three replicates were conducted independently. (c) Flowering time analysis of the indicated transgenic plants under LD. Two independent transgenic lines were analyzed. * indicated significant difference from wild type (Student’s t-test, p < 0.05). Three biological replicates were performed with at least 15 plants in total.