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. 2020 Mar 24;11(3):343. doi: 10.3390/genes11030343

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Antifungal assay of AhGLK1b-overexpressing Arabidopsis lines. (a) Schematic diagram of overexpression vector with the p35S promoter. (b) AhGLK1b-5 and AhGLK1b-6 along with Col-0. Arabidopsis lines were inoculated with equal size (about 2 mm in diameter) of S. sclerotiorum agar plug. Forty plants were infected by fungus for each line. Photographs were taken 36 hpi. Note severe chlorosis on Col-0, almost all leaf is decayed while AhGLK1b-5 and AhGLK1b-6 lines has less chlorosis indicating stronger resistance to a fungal pathogen. (c) Percent decaying lesion size, measured and calculated using ImageJ software.