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. 2020 Feb 27;9(3):639. doi: 10.3390/jcm9030639

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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MALDI -TOF and Western blot investigation of the molecular weight of the urine soluble CDH1. (A) Urine samples, where the ELISA analysis revealed high level of CDH1 were used for immunoprecipitation and subsequent mass spectrometric analysis. The MALDI-TOF analysis was performed in linear mode with sinapinic acid as matrix. The mass spectra revealed that in contrast to the full length CDH1 (MW = 124 kDa), the urine soluble one has a lower molecular weight (MW = 80 k Da). (B) The Western Blot analysis confirmed the mass spectrometric data and showed a protein with MW = 80 kDa when tested with antibody against CDH1. (C) The Western blot analysis of samples from the flow-up study confirmed the increase in CDH1 urine level toward nephropathy and highlight the prognostic value of CDH1 for diabetic nephropathy.