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. 2020 Apr 1;11:464. doi: 10.3389/fimmu.2020.00464

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Copyright © 2020 Collins, Yi, Dayuha, Whiteaker, Ochs, Freeman, Su, Paulovich, Segundo, Torgerson and Hahn.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Results of blinded sample Immuno-SRM analysis conducted by alternate laboratory. Complete process replication included DBS card punching, extraction, digestion, enrichment and MS analysis. Normal Controls (n = 25) and patients (n = 11) are compared against the diagnostic cutoff established by SCRI. Signature peptide values for WASP 274 (A) and CD42 128 (B), BTK 407 (D), DOCK8 1272 (E), and ADA 93 (F) are shown in normals and PIDD patients. Comparative diagnosis shows agreement in patient identification (C). FH, Fred Hutchinson Cancer Research Institute; SCRI, Seattle Children's Research Institute. **p < 0.01, ***p < 0.005, ****p < 0.001.