Table 3.
Analytical figures of merit for standard PIDD gradient.
| Marker type | Protein | Peptide | LLOD (fmol) | LLOQ (fmol) | ULOD (fmol) | Intra-assay CV (Average, %) | Inter-assay CV (%) | Relative difference (RT, %) | Relative difference (37°C, %) |
|---|---|---|---|---|---|---|---|---|---|
| Primary | WASP | WASP 274–288 | 5.6 | 9.4 | 937.5 | 9.7 | 9.7 | 11.6 | −9.8 |
| Primary | BTK | BTK 407–417 | 0.8 | 1.9 | 937.5 | 9.8 | 5.2 | 15.2 | −18.9 |
| Primary | CYBB | CYBB 509–521 | 4.2 | 7.5 | 7500.0 | 5.3 | 4.4 | 9.9 | −19.0 |
| Primary | ADA | ADA 93–101 | 0.8 | 3.8 | 3750.0 | 7.9 | 13.5 | 15.8 | 18.4 |
| Primary | DOCK8 | DOCK8 1272–1283 | 1.1 | 4.7 | 468.8 | 15.3 | 18.4 | 7.9 | −9.6 |
| Secondary | CD42 | CD42 128–137 | 17.6 | 30.0 | 15000.0 | 5.4 | 10.9 | 25.5 | 27.9 |
| Secondary | CD42 | CD42 154–165 | 1.1 | 7.5 | 7500.0 | 6.1 | 5.8 | 14.0 | −10.7 |
| Secondary | CD56 | CD56 122–130 | 2.7 | 3.8 | 1875.0 | 10.3 | 9.7 | 17.5 | 2.9 |
Primary markers are used for direct diagnosis of the associated PIDDs. Secondary markers provide information related to markers of hematopoiesis associated targets.