Table 4.
Comparison of LLOD, LLOQ, and CV of the Standard 20-min and HT 2.5-min Gradients.
| Marker type | Peptide | LLOD (fmol) | LLOQ (fmol) | Intra-assay CV (Average, %) | Inter-assay CV (%) | ||||
|---|---|---|---|---|---|---|---|---|---|
| 20 min | 2.5 min | 20 min | 2.5 min | 20 min | 2.5 min | 20 min | 2.5 min | ||
| Primary | WASP 274–288 | 5.6 | 5.3 | 9.4 | 18.8 | 9.7 | 19.6 | 9.7 | 9.8 |
| Primary | BTK 407–417 | 0.8 | 0.5 | 1.9 | 18.8 | 9.8 | 13.2 | 5.2 | 6.9 |
| Primary | CYBB 509–521 | 4.2 | 0.6 | 7.5 | 7.5 | 5.3 | 7.6 | 4.4 | 3.2 |
| Primary | ADA 93–101 | 0.8 | 6.9 | 3.8 | 3.8 | 7.9 | 11.6 | 13.5 | 13.2 |
| Primary | DOCK8 1272–1283 | 1.1 | 1.6 | 4.7 | 18.8 | 15.3 | 29.5 | 18.4 | 11.0 |
| Secondary | CD42 128–137 | 17.6 | 9.9 | 30.0 | 150.0 | 5.4 | 12.7 | 10.9 | 5.0 |
| Secondary | CD42 154–165 | 1.1 | 2.0 | 7.5 | 15.0 | 6.1 | 7.2 | 5.8 | 4.5 |
| Secondary | CD56 122–130 | 2.7 | 5.4 | 3.8 | 3.8 | 10.3 | 12.5 | 9.7 | 11.0 |
Primary markers are used for direct diagnosis of the associated PIDDs. Secondary markers provide information related to markers of hematopoiesis associated targets.