Table 7.

Immuno-SRM analytical validation at FHCRC including lower limits of quantification (LLOQ), Upper limit of detection (ULOD), and Intra- and Inter-assay Co-efficients of Variation (CV).

Protein	Peptide	LLOQ (fmol)	ULOD (fmol)	Intra-assay CV (Average, %)	Inter-assay CV (%)
WASP	WASP 274–288	0.24	375	9.0	6.8
BTK	BTK 407–417	0.24	375	11.0	7.1
ADA	ADA 93–101	0.96	1,500	12.8	31.5
DOCK8	DOCK8 1272–1283	0.3	187.5	11.8	10.3
CD42	CD42 128–137	3.84	6,000	11.5	5.8
CD42	CD42 154–165	1.92	3,000	16.5	13.4
CD56	CD56 122–130	0.48	750	13.1	7.2