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. 2020 Mar 9;9(3):730. doi: 10.3390/jcm9030730

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Confocal laser scanning microscopy evaluation of antibacterial efficacy of CSnp/CMCS and CSnp/DIW versus conventional chemicals on 2- and 6-week-old E. faecalis biofilm showing percentage of live bacteria. Different characters above bars indicate statistically significant difference between the groups (p < 0.01). (B) Confocal images of live/dead stained bacteria in root canal dentinal tubules of untreated and treated 6-week-old E. faecalis biofilm (green color: live bacteria, red color: dead bacteria, 40× magnification, 1 unit = 11.52 μm), the yellow line represents the interface between root canal lumen and the dentinal tubules in root canal wall.