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. 2020 Mar 18;9(3):827. doi: 10.3390/jcm9030827

Figure 1.

Figure 1

Characterization of urine stem cells (USCs). (A) Scheme of USC isolation. (B) Morphology of USCs from different donors after isolation (USC-1, 32-year-old male; USC-2, 50-year-old male; USC-3, 24-year-old male; USC-4, 22-year-old female; USC-5, 15-year-old female; USC-6, 20-year-old male). Scale bar: 400 µm. (C) Number of USCs at 14 days in the 6 urine samples. (D) Representative flow cytometric analysis of USC populations. (E) RT-PCR analysis of fibroblast markers (vimentin, twist1, fibronectin), epithelial markers (E-cadherin, claudin 1, occludin), renal epithelial markers (SLC2A1, L1CAM, NR3C2), and urothelial markers (CK13, CK20, UPK1a, UPK3a). (F) RNA sequencing of USCs, adipose derived stem cells (ADSCs), and Wharton’s jelly-derived mesenchymal stem cells (WJ-MSCs). Heatmap of hierarchical clustering of DEGs between of ADSCs, WJ-MSCs, and USCs (Fold change ≥2, p-value ≤ 0.05). (G) Dendrogram of hierarchical clustering group analysis based on gene expression profiles to show the similarities among the USCs, ADSCs, and WJ-MSCs.