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. 2020 Mar 18;9(3):827. doi: 10.3390/jcm9030827

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Y-27632 and Matrigel enhances the properties of USCs. (A) Growth curve of gelatin, gelatin + Y-27632, Matrigel, Matrigel + Y-27632 treated USCs at different time points. (B) Wound healing assay. Cell migration was assessed by the recovery of the scratch. Gelatin, gelatin + Y-27632, Matrigel, Matrigel + Y-27632 treated USCs, respectively. Scale bar: 200 µm. (C) Colony forming unit fibroblast (CFU-F) assays. At each group, representative plates of CFU-F colonies stained with crystal violet are shown (left panel). CFU-F colonies were counted after culture for 14 days. Asterisks indicate significant differences. The data are presented as fold changes compared with the gelatin group (right panel). n = 3 biological samples. (* p < 0.05, ** p < 0.01, *** p < 0.001).