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. 2020 Mar 12;5(5):e130655. doi: 10.1172/jci.insight.130655

Figure 2. PP2A is important for B cell activation and Ig production in vitro.

Figure 2

(A) Western blot analysis of PP2Aa and PP2Ac subunit expression in isolated B cells from the indicated mice. Quantification of PP2Ac expression in isolated B cells from the indicated mice. (B) ELISA analysis of the indicated serum Ig levels from the indicated mice (12–24 weeks old) (n = 3 mice per group for 2 independent experiments). (C and D) Splenic B cells were isolated from the indicated mice and were stimulated with either CpG or anti-CD40 in the presence of IL-4 for 72 hours. (C) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells with CpG and IL-4 stimulation. Middle: Dot plots represent the percentages of IgG+CD138+ plasma cells in total cultured B cells in the presence of CpG plus IL-4. Right: qPCR analysis on the expression of the indicated gene expression by the indicated B cells (n = 3 per group for 2 independent experiments). (D) Left: ELISA analysis of the indicated Ig produced by in vitro cultured B cells after anti-CD40 and IL-4 stimulation. Middle: Dot plots indicate the percentages of IgG+CD138+ plasma cells in total B cells cultured with anti-CD40 and IL-4. Right: qPCR analysis of indicated genes in the indicated B cells (n = 3 per group for 2 independent experiments). Paired t test, mean ± SEM.