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. 2020 Mar 12;5(5):e130655. doi: 10.1172/jci.insight.130655

Figure 3. PP2A is critical for B cell activation, differentiation, and Ig production in vivo.

Figure 3

(A) Dot plots show the percentages of spontaneous germinal center B cells (GC-CD19+FAS+PNA+) and T follicular helper cells (Tfh-CD4+CXCR5hiPD1hi) in spleens of the indicated 24-week-old mice (n = 6 mice per group for 2 independent experiments). (B and C) Mice were immunized i.p. with 0.2 mL/mouse SRBC. (B) IHC staining of frozen spleen sections of the indicated mice (n = 3 mice per group in 2 independent experiments). Bar graphs show the quantification (percentage) of follicles with germinal centers in total splenic follicles from the indicated mice (n = 3 mice per group in 2 independent experiments). (C) Flow cytometry analysis (%) of germinal center B cells (GC-CD19+FAS+PNA+), T follicular helper cells (Tfh-CD4+CXCR5hiPD1hi), and IgG plasma cells (PC-CD19+IgG+CD138+) in the spleens of the indicated mice after SRBC immunization. (D and E) Indicated mice were i.p. immunized with 100 μg/mouse NP-Ficoll for 5 days or 100 μg/mouse NP-CGG in 5% alum for 14 days. (D) ELISA analysis for high affinity (NP-7) or low affinity (NP-41) for NP antigen-specific IgM in the serum of 12-week-old flox/flox mice compared with control mice 5 days after immunization with T-independent antigen NP-Ficoll (n = 3 per group in 2 independent experiments). (E) ELISA analysis of high affinity (NP-7) or all affinity (NP-41) NP antigen-specific IgG in the serum of 12-week-old flox/flox mice compared with control mice 14 days after immunization with T-dependent antigen NP-CGG (n = 3 per group in 2 independent experiments). Paired t test, **P < 0.01, ***P < 0.05, mean ± SEM.