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. 2020 Mar 12;5(5):e132364. doi: 10.1172/jci.insight.132364

Figure 5. Mice with double deletion of PERK and ATF6α in Schwann cells showed normal myelination in the PNS.

Figure 5

(A) Real-time PCR analysis showed that the mRNA level of PERK was significantly reduced in the sciatic nerve of PERK-KO mice and double-KO mice compared with WT mice and that ATF6α mRNA was undetectable in the sciatic nerve of ATF6α-KO mice and double-KO mice. (B and C) PCR analysis showed that XBP1s was undetectable in the sciatic nerve of WT mice (lane 1), PERK-KO mice (lane 2), and ATF6α-KO mice (lane 3) at P21 and P45 but became modestly detectable in double-KO mice (lane 4) at P21 and P45. (D–G) Toluidine blue staining showed normal myelination in the sciatic nerve of 21-day-old double-KO mice compared with WT mice, PERK-KO mice, and ATF6α-KO mice. (H–K) Toluidine blue staining showed normal myelination in the sciatic nerve of 45-day-old double-KO mice compared with WT mice, PERK-KO mice, and ATF6α-KO mice. Scale bars: 10 μm. N = 4 animals. ND, not detected. Error bars represent mean ± SD. Statistical analyses were done with a 1-way ANOVA with Tukey’s posttest; *P < 0.05.