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. 2020 Mar 12;5(5):e135449. doi: 10.1172/jci.insight.135449

Figure 2. Differences in ID in the early IgM response between newborn and adult AGMs following IAV infection.

Figure 2

To measure Abs against each of the identified neutralizing HA head epitopes, a panel of Δ4 HA constructs were used, each of which had mutations in 4 of the 5 antigenic sites. The name designates the remaining epitope. IgM binding to each construct was assessed at day 8 (A) or day 14 (B) following PR8 infection (n = 4/group). The starting dilution of plasma was 1:10. Following AUC calculation, the percentage of the response based on the WT HA AUC was determined. Significance was determined by 1-way repeated-measures (RM) ANOVA with a Holm-Šídák test for multiple comparisons. Significant differences in binding between epitopes are denoted by lowercase letters corresponding to an index capital letter, e.g., “A” versus “a,” where values with the “a” are significantly different (threshold P < 0.05) from those with the “A.”