(A) RT-qPCR of Aim2 mRNA in FACS-isolated gastric immune subpopulations from 6-month H. felis–infected WT (blue) versus Aim2–/– (pink) stomachs. n = 3. (B) Microarray heatmap of FACS-isolated B cells from 6-month H. felis–infected WT versus Aim2–/– gastric mucosa. (C) Plot of FACS-quantified percentages of homing CD62L– splenic CD8+ T cells following treatment with recombinant GDF11, CXCL16, CCL8, or CXCL9 with or without prior anti-CD3/anti-CD28 activation. Each data point represents 1 experiment. (D) Representative FACS plot showing CD62L+ versus CD62L– splenic CD8+ T cells in recombinant CXCL16-treated versus untreated, with or without prior activation with anti-CD3/anti-CD28. (E) RT-qPCR analysis of CXCL16 mRNA expression in the stomach from 6-month H. felis–infected WT, Aim2–/–, and IFNAR1–/– versus Aim2–/– IFNAR1–/– mice. Each data point represents 1 mouse. Error bars represent the mean ± SEM. N.S., not significant; *P < 0.05; **P < 0.01. Data were compared using one-way ANOVA with Dunnet’s (parametric) multiple comparison tests.