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. 2020 Mar 12;5(5):e134539. doi: 10.1172/jci.insight.134539

Figure 4. ApoE does not affect lung myofibroblast differentiation.

Figure 4

(A) Human lung Fb MRC5 were treated with 2 ng/mL TGF-β1 together with or without 1 μg/mL human ApoE for 48 hours. Total RNAs were isolated and levels of the indicated genes determined by real-time PCR. (B) MRC5 cells were treated with 2 ng/mL TGF-β1 in conditioned media (CM) with or without ApoE. Forty-eight hours after treatment, cells were collected and protein levels of indicated genes determined by Western blotting. n = 3 for each group. (C and D) MRC5 cells were treated with 2 ng/mL TGF-β1 for 48 hours (1st Tβ1) to induce myofibroblast differentiation. Myofibroblasts were washed 3 times and recultured in fresh control media or TGF-β1–containing media (2nd Tβ1) with or without 1 μg/mL ApoE (C), or conditioned media with or without ApoE (D) for additional 48 hours. Total RNAs were isolated and levels of the indicated genes determined by real-time PCR. n = 3–4 for each group; mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001 by 1-way ANOVA with Bonferroni’s post hoc test.