. Author manuscript; available in PMC: 2020 Apr 8.

Published in final edited form as: J Biomol NMR. 2019 Aug 12;73(8-9):493–507. doi: 10.1007/s10858-019-00258-0

Table 2.

Isomerization rates for LRT2 mutants and homologs determined by ¹H-¹H ROESY and ¹H-¹⁵N ZZ exchange experiments.

CyclophilinVariant	ROESY-measured $k_{e x}^{o b s}$ (s⁻¹) 120μM LRT2 1.84μM OsIAA11^98–109	Nzz-measured $k_{e x}^{o b s}$ (s⁻¹) 16μM LRT2 800μM OsIAA11^72–125
LRT2 (WT)^a	3.63±0.08^a	0.62±0.01^a
LRT2 R62A	ND^b	Not measured
LRT2 P125K	2.7±0.3	0.47±0.04
LRT2 W128A	ND^b	0.0±0.04
LRT2 H133Q	ND^b	0.12±0.04
LRT2 VPCS	3.3±0.3	0.55±0.04
hCypA	Not measured	0.69±0.04
PPIB	Not measured	0.37±0.04
TaCypA	1.8±0.3	Not measured

Data reported previously¹⁶.

Could not be determined due to the absence of exchange crosspeaks at all mixing times.