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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Adv Biosyst. 2019 Oct 1;3(12):1900141. doi: 10.1002/adbi.201900141

Figure 5.

Figure 5.

Localized BMP-2 delivery from HA nanoparticles promotes osteogenic differentiation throughout the spheroid. (A) Schematic of MSC spheroid induction with BMP-2 adsorbed onto HA. (B) IBSP expression in MSC spheroids as a function of BMP-2 and HA treatment after 7 days in osteogenic culture (n=4). (C) ALP activity in MSC spheroids at day 7 (n=3). (D) Representative histology demonstrating picrosirius red (top) and osteocalcin staining (bottom) of spheroids at day 14. Scale bars are 200 μm. Groups with different letters are statistically different based on a one-way ANOVA with p<0.05. Data are mean ± standard deviation.