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. Author manuscript; available in PMC: 2020 Dec 1.
Published in final edited form as: Adv Biosyst. 2019 Oct 1;3(12):1900141. doi: 10.1002/adbi.201900141

Figure 6.

Figure 6.

Localized BMP-2 delivery by HA enhances the retention of the osteogenic phenotype after removal of soluble osteogenic cues. (A) Schematic of studies to evaluate osteogenic retention in spheroids. (B) Total calcium after 5 days in growth media (n=3). (C) The percent change in calcium from osteogenic induction to 5 days in media lacking additional osteogenic supplements (n=3). (D) RUNX2 expression in MSC spheroids as a function of BMP-2 and HA treatment compared to expression in OM after 5 days in media lacking additional osteogenic supplements (n=4). (E) Representative histology of picrosirius red (top) and osteocalcin staining (bottom) in spheroids after 5 days in growth media. Scale bars are 200 μm. Groups with different letters are statistically different based on a one-way ANOVA with p<0.05. Data are mean ± standard deviation.