Figure 2.
Three-chamber social approach deficits in Pten conditional heterozygous mice are corrected in Pten and Mtor double conditional heterozygous mice. (A) Graph showing percent time female mice spent in each chamber. Paired t-tests were used to compare time spent in the chambers containing a mouse in a tube (Mouse chamber, M) and an empty tube (Object chamber, O) separately for each genotype. *P < 0.05, and **P < 0.01. n.s. indicates no significant difference. (B) Example heat maps showing the time spent in each chamber. M indicates mouse chamber, and O indicates object chamber. (C) Graph showing the velocity of the mice during the 10 min sociability test. Velocity is not altered across genotypes. One-way ANOVA was used. F(3,41) = 0.32, P = 0.81. N = 12 Control, 12 Emx1-Cre+; Ptenloxp/+, 9 Emx1-Cre+; Ptenloxp/+; Mtorloxp/+, and 12 Emx1-Cre+; Ptenloxp/+; Ctnnb1 loxp/+ mice.