Figure 3.

Increased vGluT1 puncta in Pten conditional heterozygous mice is corrected in Pten and Mtor double conditional heterozygous mice. (A) Cartoon and an image showing the staining of the vesicular glutamate transporter 1 (vGluT1) and the vesicular GABA transporter (vGAT) in the prelimbic cortex (PrL). vGluT1 and vGAT puncta were quantified separately in outer and inner layer 1. Scale bar: 50 μm. (B) Representative images of vGluT1 and vGAT staining in the PrL of each genotype. Scale bar: 10 μm. (C–F) Quantification of vGluT1 and vGAT puncta in outer (C and D) and inner (E and F) layer 1 of PrL. N = 4 animals per genotype. (G) Cartoon and an image showing the staining of the vGluT1 and vGAT in the basolateral amygdala (BLA). Scale bar: 50 μm. (H and I) Quantification of vGluT1 (H) and vGAT (I) puncta in the BLA. N = 4 animals each genotype. (J) Representative images showing fluorescent Nissl staining of layerV cells in the primary somatosensory cortex. Scale bar: 50 μm. (K) Quantification of layer V cell soma size. N = 5 animals per genotype. For all quantification graphs, one-way ANOVA was used, and Tukey’s post hoc tests were used where appropriate. *P < 0.05, and **P < 0.01. n.s. indicates no significant difference. F(3, 12) = 8.25, P = 0.003 (C); F(3, 12) = 1.01, P = 0.421 (D); F(3, 12) = 0.641, P = 0.603 (E); F(3, 12) = 0.337, P = 0.799 (F); F(3, 12) = 1.95, P = 0.176 (H); F(3, 12) = 1.94, P = 0.177 (I); F(3, 16) = 7.986, P = 0.002 (K).