Fig. 6.

Molecular mechanism for the assembly of adhesome complexes in airway smooth muscle: (1) ACh stimulation activates RhoA, which regulates regulatory light chain phosphorylation of NM myosin and the assembly and activation of NM myosin II. (2) Activated NM actomyosin mediates the recruitment of inactive proteins to membrane adhesome complexes, where they undergo activation to regulate cytoskeletal signaling pathways. (3) Cdc42 activates N-WASp and the Arp2/3 complex, which catalyzes cortical actin polymerization. Signals from G-protein coupled receptors (GPCR) activate MLC kinase which regulates SM myosin regulatory light chain phosphorylation and the activation of SM myosin crossbridge cycling.