Table 1. Experimental groups and study design.
| Primary infection | 15 month interval | Secondary infection | ||||||
| Group / Infection Method | N | Virus† | Inoculum per animal# | Group / Infection Method | N | Virus† | Inoculum per animal# | |
| SC | 10 | DENV-1 | 5.18 log10 PFU | SC D1-D1 | 5 | DENV-1 | 5.18 log10 PFU | |
| SC D1-D2 | 5 | DENV-2 | 5.18 log10 PFU | |||||
| ID | 9 | DENV-1 | 5.18 log10 PFU | SC N-D2 | 5 | DENV-2* | 5.18 log10 PFU | |
| ID+SGE | 10 | DENV-1 | 5.18 log10 PFU + 15 SGeq | IMF N-D2 | 5 | DENV-2* | 15 mosquitoes | |
| IMF | 10 | DENV-1 | 15 mosquitoes | IMF D1-D1 | 5 | DENV-1 | 15 mosquitoes | |
| IMF D1-D2 | 5 | DENV-2 | 15 mosquitoes | |||||
*primary DENV2 infection controls for the SC injection and IMF methods
†DENV-1 strain 0111/2011; DENV-2 strain 0126/2010
#IMF animals were exposed to the probing and/or feeding of 15 mosquitoes each; details of the inoculum are available in the “Materials and Methods” and in S4 Table.
SC = subcutaneous; ID = intradermal; SGE = salivary gland extract; IMF = infectious mosquito feeding; SGeq = salivary gland equivalents in PBS