Fig 1. Localization of H3K4me2 (1–1), H3K4me3 (1–2), H3K27ac (1–3), H3K79me2 (1–4), and H3K79me3 (1–5) in male germline cells in the young (3 M) testis.

(a) Representative confocal images of histone marks (magenta) and SCP3 (green) in the seminiferous epithelium in stages I, V, VIII, X and XII. Nuclei were counterstained with DAPI (blue). The numbers with an arrowhead indicate an individual cell at the specific stage during spermatogenesis, as shown in Table 2. Scale bar: 20 μm. Negative controls (without primary antibodies) are shown in Fig 1–1. (b) Magnified images of the cells indicated by arrowheads in (a), and subcellular localization of each histone modification in the young testis is shown in magenta in a graphical summary. The upper panels are merged images of histone marks (magenta) and DAPI (blue), and the lower panels are grayscale images for the histone marks. Each parenthesis represents the stage of spermatogenesis (see Table 2.).