Table 3. The number of colonies which can be included is a GWAS for a given budget, in the alternative approaches.
| Colonies | Samples | Extraction cost | Libraries | Library prep cost | Sequencing cost | Total cost | ||
|---|---|---|---|---|---|---|---|---|
| WG- seq | Individual seq | 50 | 300 | 1,500 | 300 | 9,000 | 9,000 | 19,500 |
| Normalized pool-seq | 115 | 1,147 | 5,735 | 115 | 3,441 | 10,324 | 19,500 | |
| Un-normalized pool-seq | 156 | 156 | 780 | 156 | 4,680 | 14,040 | 19,500 | |
| RAD-seq | Individual seq | 197 | 1,182 | 5,909 | 1,182 | 11,818 | 1,773 | 19,500 |
| Normalized pool-seq | 302 | 3,023 | 15,116 | 302 | 3,023 | 1,360 | 19,500 | |
| Un-normalized pool-seq | 1,000 | 1,000 | 5,000 | 1,000 | 10,000 | 4,500 | 19,500 |
Cost estimates in USD. Assuming 5 USD per DNA extraction; 30 USD per WG library construction and 10 per RAD library; 10 USD per Gb sequencing; 3 Gb for a genome depth of 10X per individual sample or 9 Gb for 30X per pool; and 20-fold less sequencing for RAD-seq relative to WG-seq.