Table 1. Primer sequences for target genes.
| Target | Gene symbol | Sequence (5’ to 3’) | Annealing temperature°C | Product length (bp) | |
|---|---|---|---|---|---|
| EF1A | LOC100136525 | F | AGGCTGCTGAGATGGGTAAG | 63 | 218 |
| R | AGCAACGATAAGCACAGCAC | ||||
| NKA a1b (ii) | LOC 106575572 | F | GGGTGTGGGCATCATTTCTG | 66 | 152 |
| R | CATCCAACTGTTCGGCTGAC | ||||
| CFTR I | LOC 100136364 | F | CCTTCTCCAATATGGTTGAAGAGGCAAG | 63 | 81 |
| R | GCACTTGGATGAGTCAGCAG | ||||
| CAPN2 | LOC 106589985 | F | GTTGAGGAGATCGTGGTGGA | 65 | 118 |
| R | TGTTCAGAATCCTCCGCAGT | ||||
| TPH1 | LOC 106562311 | F | ACTTCCTCAGAGAACGCACA | 63 | 218 |
| R | CTGGGAGAACTGGGCAAAAC | ||||
| S100A1 | LOC 106570104 | F | GGATGACCTGATGACGATGC | 65 | 122 |
| R | ATCACATACTCCCCACCAGG | ||||
| ST6GALNAC2 | LOC 106589898 | F | CTTCGACCGCCAATATCACC | 63 | 149 |
| R | ATGGCAACCTTGAGTGAGTT | ||||
| FKBP5 | LOC 106565346 | F | CTGGGAAAGGGTCAGGTGAT | 65 | 264 |
| R | GACTGTTGATCCGTCGTTGG | ||||
| SLC5A7 | LOC 106602131 | F | AGGTGGGACGTGTTTCAGAT | 65 | 203 |
| R | CCCGACCAACAAAACCCTTT |