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. Author manuscript; available in PMC: 2020 Apr 8.
Published in final edited form as: Radiat Res. 2018 Aug 22;190(5):538–557. doi: 10.1667/RR15099.1

FIG. 1.

FIG. 1.

Sensitivity to ionizing radiation in H460wt and H460crp53 cells. Panel A: Clonogenic survival assay. H460wt and H460crp53 cells were exposed to the indicated doses and incubated for 11 days prior to determination of colony formation. Panel B: Cell viability. Cells were exposed to 6 Gy, incubated with fresh media for the indicated number of days and viable cell number determined by trypan blue exclusion. Panel C: Apoptosis time course. After 6 Gy irradiation, apoptotic cells were identified by annexin V/PI staining and flow cytometry at the indicated days. Panel D: Apoptosis dose response. After irradiation at the indicated doses, cells were incubated with fresh media for two days and apoptotic cells were identified by annexin V/PI staining and flow cytometry. Results are from three independent experiments. *P < 0.05, irradiated H460wt vs. H460crp53 cells.