TABLE 1.
Resistance-Associated Proteins and Senescence-Associated Secretory Phenotype (SASP) Factors in the Secretome of H460wt and H460crp53 Cells
| Control | Irradiated | |||||||
|---|---|---|---|---|---|---|---|---|
| Protein accession | Protein name | Gene name | N: Sequence coverage (%) | N: Mol. weight (kDa) | −Log10 (P value) | Difference | −Log10 (P value) | Difference |
| Resistance-associated proteins | ||||||||
| P08670 | Vimentin | VIM | 46.8 | 53.651 | 0.2595 | 0.5584 | 3.1221 | −1.5103 |
| P63000 | Ras-related C3 botulinum toxin substrate 1 | RAC1 | 43.2 | 21.45 | 0.0769 | 0.1594 | 3.1066 | −1.2153 |
| Q9NSK0 | Kinesin light chain 4 | KLC4 | 8.4 | 62.506 | 0.1875 | −0.3751 | 2.1090 | −2.1474 |
| O60568 | Procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 | PLOD3 | 32 | 84.784 | 0.1275 | −0.1237 | 1.7999 | 0.7549 |
| P36952 | Serpin B5 | SERPINB5 | 22.7 | 42.1 | 0.0163 | 0.0218 | 1.5157 | −0.5508 |
| P49327 | Fatty acid synthase | FASN | 40.3 | 273.42 | 0.5180 | −0.9924 | 1.4559 | −0.4168 |
| P27695 | DNA-(apurinic or apyrimidinic site) lyase | APEX1 | 46.2 | 35.554 | 0.6547 | −0.9087 | 1.0257 | −0.4148 |
| P08758 | Annexin A5 | ANXA5 | 23.1 | 35.936 | 0.4295 | 0.3975 | 1.0061 | 1.0789 |
| P31947 | 14-3-3 protein sigma | SFN | 48.8 | 27.774 | 0.6745 | −0.8959 | 0.9709 | −0.9161 |
| P15531 | Nucleoside diphosphate kinase A | NME1 | 65.1 | 17.149 | 0.0049 | 0.0068 | 0.5840 | −0.1710 |
| P62491 | Ras-related protein Rab-11A | RAB11A | 29.8 | 24.488 | 0.2692 | −0.3305 | 0.5442 | −0.1878 |
| Q5JPE7 | Nodal modulator 2 | NOMO2 | 6.2 | 139.38 | 0.1650 | −0.1895 | 0.5106 | 0.4930 |
| P31930 | Cytochrome b-c1 complex subunit 1, mitochondrial | UQCRC1 | 5.4 | 52.645 | 0.3140 | −0.7046 | 0.4073 | 0.5895 |
| P30040 | Endoplasmic reticulum resident protein 29 | ERP29 | 30.7 | 28.993 | 0.4358 | 1.6002 | 0.0920 | 0.0590 |
| P62937 | Peptidyl-prolyl cis-trans isomerase A | PPIA | 67.3 | 18.012 | 0.1148 | 0.5008 | 0.0495 | −0.0518 |
| Senescence-associated secretory proteins | ||||||||
| P09341 | Growth-regulated alpha protein | CXCL1 | 45.8 | 11.301 | 4.1403 | 5.3441 | 5.3041 | 3.7295 |
| P05231 | Interleukin-6 | IL6 | 42.9 | 21.494 | 0.6601 | 1.2458 | 3.6702 | 3.7453 |
| P42830 | C-X-C motif chemokine 5 | CXCL5 | 43 | 11.972 | 0.0768 | 0.3904 | 3.4760 | 3.1293 |
| P55268 | Laminin subunit beta-2 | LAMB2 | 26.1 | 195.98 | 1.9894 | 1.2477 | 3.3122 | 0.6565 |
| P03956 | Interstitial collagenase | MMP1 | 11.1 | 54.006 | 0.5693 | 0.5751 | 3.2670 | 1.0677 |
| O15230 | Laminin subunit alpha-5 | LAMA5 | 28 | 399.73 | 0.7360 | 0.5856 | 2.8928 | 1.0147 |
| P17936 | Insulin-like growth factor-binding protein 3 | IGFBP3 | 53.2 | 28.98 | 0.4682 | −0.7370 | 2.6325 | 1.4437 |
| P22692 | Insulin-like growth factor-binding protein 4 | IGFBP4 | 34.9 | 27.934 | 0.5261 | 1.8182 | 2.5945 | 1.0343 |
| P19876 | C-X-C motif chemokine 3 | CXCL3 | 34.6 | 11.342 | 0.0594 | 0.0906 | 2.5362 | 4.2024 |
| P07942 | Laminin subunit beta-1 | LAMB1 | 34.1 | 200.48 | 1.8644 | 1.3326 | 2.3580 | 0.6935 |
| P25391 | Laminin subunit alpha-1 | LAMA1 | 32.6 | 337.08 | 0.7519 | 1.2152 | 2.1027 | 0.7547 |
| P16035 | Metalloproteinase inhibitor 2 | TIMP2 | 18.6 | 24.399 | 0.7339 | −0.9250 | 2.0269 | 0.9077 |
| P24592 | Insulin-like growth factor-binding protein 6 | IGFBP6 | 15.8 | 25.322 | 1.0006 | −1.9892 | 1.5490 | 0.7319 |
| Q16270 | Insulin-like growth factor-binding protein 7 | IGFBP7 | 24.8 | 29.13 | 0.6870 | −1.0294 | 1.4286 | 0.1357 |
| P03950 | Angiogenin | ANG | 19.7 | 16.55 | 0.2687 | −0.3723 | 1.2610 | −2.0546 |
| P01033 | Metalloproteinase inhibitor 1 | TIMP1 | 52.7 | 23.171 | 0.7765 | 2.9735 | 1.2018 | 0.7942 |
| P07858 | Cathepsin B | CTSB | 36 | 37.821 | 0.6240 | 2.1588 | 1.1950 | 0.6746 |
| P02751 | Fibronectin | FN1 | 42.7 | 262.62 | 1.0938 | 1.3290 | 1.0122 | 0.7676 |
| P11047 | Laminin subunit gamma-1 | LAMC1 | 48 | 177.6 | 1.1392 | 0.6569 | 0.7737 | 0.4710 |
| P18065 | Insulin-like growth factor-binding protein 2 | IGFBP2 | 46.8 | 34.814 | 0.1856 | −0.4689 | 0.7302 | 0.4093 |
| P14174 | Macrophage migration inhibitory factor | MIF | 11.3 | 12.476 | 0.0951 | 0.2367 | 0.3994 | 0.3085 |