Figure 1:

Experimental setups to characterize DMI (A) on phantoms in vitro, (B) on rat brain post mortem and (C) on human brain in vivo. Due to the lack of a ¹H MRI coil, all images were acquired in a separate study and are shown for illustration purposes only. (A) Phantoms with (R3.9) different KCl concentrations are used to change sample conductivity, and hence achieve a range of coil loads in vitro, whereby signal is always acquired from a 2 mm slice 6 mm below the ²H RF coil. (B, C) On rat brain post mortem and human brain in vivo, signal is acquired in the form of 3D DMI whereby analysis is limited to the indicated slice position. On human brain a 100% D₂O phantom was used for RF power calibration and then replaced with a 0.1% D₂O phantom for position referencing during DMI. All distances are in mm.