Figure 6. MRC-5 cells stably expressing GFP-Rab5, GFP-SNX1 or stained with Lysobrite with tracking data overlaid.
The colours show the value of estimated by the neural network using a 15 point window. The scalebar is 10 µm.
Figure 6—figure supplement 1. Distribution of endogenous Rab5 and SNX1.
MRC-5 cells were fixed and labeled with antibodies to Rab5 and SNX1, then imaged by confocal microscopy. A maximum-intensity z-projection of deconvolved images is shown. The boxed region is enlarged and presented as grey-scale single channels and a two color merged image. The scale bar is 10 µm (main image) and 2 µm (enlargements).
Figure 6—video 1. Video of MRC-5 cell stably expressing GFP-Rab5.
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The field of view is 62.88 µm by 62.88 µm (600 × 600 pixels) and playback is in real time.
Figure 6—video 2. Video of MRC-5 cell stably expressing GFP-SNX1.
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The field of view is 62.88 µm by 62.88 µm (600 × 600 pixels) and playback is in real time.
Figure 6—video 3. Video of MRC-5 cell stained with Lysobrite.
Download video file (3.2MB, mp4)
The field of view is 62.88 µm by 62.88 µm (600 × 600 pixels) and playback is in real time.