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. 2020 Apr 8;6(15):eaax2746. doi: 10.1126/sciadv.aax2746

Fig. 3. The catalytic activity of LSD1 is dispensable.

Fig. 3

(A) Western blot analysis of LSD1 levels in NB4 LSD1 KO cells transduced with expression vectors encoding for wild-type LSD1 or the catalytically inactive mutant K661A-LSD1. Empty vector was used as control, and actin served as loading control. (B) Growth curves of NB4 LSD1 KO cells transduced with empty vector, LSD1, or K661A-LSD1 and treated with 0.01 μM RA (DMSO as control treatment). (C) Percentage of CD11b-expressing cells assessed by FACS in NB4 LSD1 KO cells transduced with expression vectors encoding for LSD1, K661A-LSD1, or empty vector, treated with 0.01 μM RA (or DMSO as control) for 24 hours. (D) H3K4me2 enrichment at the promoter of PI16 (left), ITGAM (center), and TGM2 (right) in NB4 and NB4 LSD1 KO cells transduced with expression vectors encoding for LSD1, K661A-LSD1, or empty vector, assessed by ChIP-qPCR. The results represent percentage of input chromatin. Error bars indicate SD from triplicate experiments, and P values were determined by a two-tailed unpaired Student’s t test (*P < 0.05, **P < 0.01, and ***P < 0.001).