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. 2020 Apr 8;6(15):eaax2746. doi: 10.1126/sciadv.aax2746

Fig. 5. The interaction of LSD1 with GFI1 is fundamental for LSD1 activity in APL cells.

Fig. 5

(A) Heat maps showing ChIP-seq signal of LSD1 and GFI1 ranked according to decrescent LSD1 signal. bp, base pairs. (B) Representative examples of overlapping LSD1 and GFI1 binding regions. University of California Santa Cruz Genome Browser profile of LSD1 and GFI1 ChIP-seq on the PI16 gene promoter and interferon regulatory factor 8 (IRF8) putative enhancer is shown. (C) HDAC1 recruitment (measured by ChIP-qPCR) at three genomic regions bound by both LSD1 and GFI1, before and after treatment with MC_2580. n.s., not significant. **P < 0.01 and ***P < 0.001. (D) Western blot analysis of LSD1 and GFI1 in GFI IP assays performed in NB4 LSD1 KO cells reconstituted with wild-type LSD1, LSD1-D553,555,556A mutant, or empty vector (as control). IgG, immunoglobulin G. (E) Growth curves of NB4 LSD1 KO cells transduced with wild-type LSD1, LSD1-D553,555,556A, or empty vector and treated for the indicated time with 0.01 μM RA (or DMSO as control).