Table 2. Genotyping PCR programs and band sizes.
| Gene | Primer name | Cycle | Band sizes |
|---|---|---|---|
| Tor1a ΔE floxed Tor1b | Lox-gtF | 94°C, 3 min; 94°C, 30 s; 61.5°C, 30 s; 72°C, 30 s, 34 cycles; 72°C, 5 min | WT – 245 bp Floxed– 336 bp |
| Lox-gtR | |||
| Frt-gtF | |||
| Frt-gtR | |||
| B-OE | Rosa-gtF | 94°C, 3 min; 94°C, 30 s; 61°C, 30 s; 72°C, 30 s, 30 cycles; 72°C, 5 min | WT – 469 bp Mutant – 188 bp |
| Rosa-gtR | |||
| Mut-R |