Table 1.

Summary of differentiation protocols

Publication		Method	Markers	Timing	Species	Ref
2D	Zhao, 2002	RA or FGF2 induction. Ectopic Crxexpression	None of RGCs	NA	mouse	38
	Lamba, 2006	IGF, Dkk1, Noggin	PAX6, HUC/D	NA	human	32
	Osakada, 2008	Dkk1, LeftyA, Activin, DAPT	PAX6, ISL1	28 days	mouse	62
	Kayama, 2010	ectopic Pax6 transfection	PAX6, ISL1, BRN3, THY1, OPN4, NFM	14 days	mouse	39
	La Torre, 2012	IGF, Dkk1, Noggin	BRN3, TUJ1	21 days	mouse and human	34
	Boucherie, 2013	Matrigel, N2, B27	None of RGCs	NA	human	63
	Riazifar, 2014	Dkk1, LeftyA, Activin, DAPT	BRN3, TUJ1, ISL1, gSYN, THY1	21 days	human	64
	Sluch, 2015	Matrigel, N2, B27	BRN3, TUJ1, NEUN, THY1, MAP2, SNC, ISL1, RBPMS, PAX6	25 days	human	66
	Teotia, 2016	SHH, FGF8, DAPT, Follistatin, Cyclopamine	BRN3, TUJ1, THY1	15 days	mouse and human	65
	Chao, 2017	IGF, Dkk1, Noggin and DAPT	BRN3, PAX6, HUC/D, TUJ1	60 days	human	67
	Daniszewski, 2018	IGF, Dkk1, Noggin	cRNAseq	36 days	human	68
3D	Eiraku, 2011a	2% Matrigel, 1.5% KSR	BRN3, PAX6, CALRET	10 days	mouse	35
	Eiraku, 2011b	2% Matrigel, 1.5% KSR	BRN3, PAX6, CALRET	13 days	mouse	60
	Nakano, 2012	2% Matrigel, 10% KSR, IWR1e	BRN3	30 days	human	36
	La Torre, 2015	2% Matrigel, 1.5% KSR	BRN3, PAX6, TUJ1	13 days	mouse	50
	Decembrini, 2014	2% Matrigel, 1.5% KSR	PAX6	20 days	mouse	69
	Volkner, 2016	2% Matrigel, 1.5% KSR, DAPT	BRN3, ELAVL3/4)	14 days	mouse	70
	Aparicio, 2017	0.5% Matrigel, 10% KSR, IWR1e	BRN3, RBPMS	27 days	human	71
	Yokoi, 2017	2% Matrigel, FBS, BDNF	BRN3, Tuj1	30 days	human	72
	Kobayashi, 2018	2% Matrigel, 1.5% KSR, RA	BRN3, THY1, ISL1, RBPMS	21 days	human	73
	DiStefano, 2018	2% Matrigel, 1.5% KSR, RA	BRN3a	15 days	mouse	74
iPSCs	Meyer, 2009	N2, B27	None of RGCs	NA	human	33
	Parameswaran, 2010	Noggin, FGF2	PAX6, BRN3b, RPF1	15 days	mouse	75
	Tucker, 2011	IGF, Dkk1, Noggin, bFGF, DAPT	NF200	33 days	mouse	76
	Tucker, 2013	IGF, Dkk1, Noggin, bFGF, DAPT	NF200, BRN3B	60 days	human	77
	Zhong, 2014	Blebbistatin, N2, B27, RA	BRN3, HUC/D	35 days	human	37
	Riazifar, 2014	bFGF, DAPT	BRN3A, BRN3B, THY1, TUJ1, ISL1, SNC	40 days	human	64
	Tanaka, 2015	2% Matrigel, FBS, BDNF	BRN3b, TUJ1, ISL1, SNCG, NFLM	34 days	human	79
	Teotia, 2016	SHH, FGF8, DAPT, Follistatin, Cyclopamine	BRN3, TUJ1, THY1	15 days	mouse and human	65
	Vergara, 2017	Blebbistatin, N2, B27, RA	BRN3b	35 days	human	80
	Yokoi, 2017	2% Matrigel, FBS, BDNF	BRN3, Tuj1	30 days	human	72
	Langer, 2018	N2, B27	RNAseq	40 days	human	81