Figure 7.

Estimation of cell number in the spiral ganglion region of the cochlea from mid-modiolar cochlear cross sections. A, DAPI-labeled nuclei were counted in the spiral ganglion region of P30 WT and KI mice from the Hgf^del10Neo (WT = 8 mice, KI = 7 mice) and Hgf^del10 (WT = 8 mice, KI = 5 mice) mouse lines. The number of DAPI-labeled nuclei did not demonstrate a statistically significant difference between WT and KI mice from either mouse line (p = NS). B, Cells in the spiral ganglion region were counted from H&E-stained mid-modiolar cross sections of P90 WT (n = 4 mice) and KI (n = 4 mice) mice from the Hgf^del10Neo mouse line. The number of cells in the spiral ganglion region between WT and KI mice did not differ significantly (p = NS). C, Representative images of DAPI-labeled mid-modiolar cross sections depicting the spiral ganglion region of both P30 WT and KI mice from both the Hgf^del10Neo and Hgf^del10 mouse lines. No visually apparent differences were noted between WT and KI mice. Scale bars, 50 μm. D, Representative images of H&E-stained mid-modiolar cross sections depicting the spiral ganglion region of both P90 WT and KI mice from the Hgf^del10Neo mouse line. No visually apparent difference was noted between WT and KI mice. Scale bars, 50 μm.