Table 1.

Sequence stability of the E1-V80X variants in mammalian and mosquito cell lines. See alsoFigure S1 to S3.

	Mammalian cells (BHK-21)		Mosquito cells (C6/36)
Amino acid Substitution	Position 80 Stability	Second site mutations	Position 80 Stability	Second site mutations
E1-V80	Stable		Stable
E1-V80G	Stable	E1-V344L and CP-V56L	Stable	E1-T207T and CP-G199G
E1-V80A	Stable		Stable
E1-V80L	Stable		Stable	E1-V226A or E1-A194V
E1-V80I	Stable		Stable	E1-T65A or E1-A66A:E1-D70N
E1-V80Q	Stable	E1-V226A	Stable or reverted	E1-G139E or E1-V226A
E1-V80N	Stable, reverted or mutated to D, S or T	E1-T82P or 1-A129M	Stable or reverted	E1-T82I or E1-A129V
E1-V80C	Stable or reverted	E1-F95F and/or E1-A129V	Reverted	E1-A129V
E1-V80M	Stable, reverted or mutated to I or A		Reverted or mutated to I or A
E1-V80F	Reverted or mutated to A	E1-A129V or E1-A129M	Reverted	E1-T65T and/or E1-A129V
E1-V80Y	Reverted or mutated to I or G		Reverted or mutated to S
E1-V80W	Reverted or mutated to I	E1-A129M	Reverted or mutated to S	E1-A129E and CP-Q166L:E2-T2T:E2-V32I
E1-V80P	Stable or mutated to S	E1-T65S or E1-A239A:E1-A359N	Mutated to S
E1-V80S	Stable or mutated to A	CP-P57L	Stable	E1-T41A
E1-V80T	Stable or mutated to N or I	E1-T82I	Stable or mutated to A	E1-V226A or CP-P137L:E2-V32I
E1-V80H	Reverted or mutated to T or S	E1-A129M	Not rescued
E1-V80R	Reverted or mutated to L or D		Reverted	E1-A129V/M/I
E1-V80K	Reverted	E1-A129V	Reverted	E1-A129V
E1-V80D	Stable or mutated to S	E1-H107Y	Stable or reverted	E1-V226A or E1-A129V
E1-V80E	Stable or mutated to A	E2-E24K	Stable or reverted	E1-V226A or E1-A342D and CP-Q166L: E2-T2T:E2-V32I