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Schematic procedure to obtain hyperacute serum (HAS)
In order to prepare HAS, blood is collected into specific tubes containing coagulation activators (VACUETTE® Z Serum C/A tubes, Greiner Bio-One International GmbH, Kremsmünster, Austria) and centrifuged. Once the fibrin clot has been formed, it is placed in a new container and squeezed to obtain the serum portion, resulting in HAS35.
