Figure 3.
Metabolic impact of supraphysiological ionic and hypertonic stress.
(A and C) Partial least square-discriminant analyses (PLS-DA) reveals significant impact of ionic stress induced by ionomycin (which induces increases in intracellular Ca2+) or hypertonic stress in red blood cells (RBCs) and supernatants, respectively. (B and D) Top 25 significant metabolites by ANOVA in RBCs and supernatants are shown. Key metabolites are highlighted in the dot plots in the centre of the figure, colour-coded as per the legend on the right-hand side of the figure.
ATP: adenosine triphosphate; G6P: glucose 6-phosphate; PS: phosphatidylserine; RibP: ribose phosphate.