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. 2020 Feb 13;39(15):3128–3144. doi: 10.1038/s41388-020-1198-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Volcano plots showing the gene expression profiles of control (left), HES5wt-inducible (middle), or HES5mut-inducible (right) Hep3B cell lines with or without 2 µg/ml Dox treatment. The x-axis specifies the log2 fold changes and the y-axis specifies the negative log10 of the limma t-test p-values. The horizontal line indicates p = 0.001. Among genes with the highest negative fold change in HES5wt-expressing cells are HES1, ODC1, and LDHA, which are depicted by red dots in the middle panel. Accordingly, protein levels of HES1 and N1ICD (b) or ODC1 and LDHA (c) were analyzed by western blotting upon HES5wt or HES5mut expression in Hep3B cells. One representative experiment out of three with similar outcome is shown and β-actin served as loading control. d Quantification of protein levels of HES1, NOTCH1, ODC1, and LDHA in Hep3B cells following HES5wt or HES5mut induction obtained from three independent western blotting experiments. *Mann–Whitney U-test p < 0.05.